12 Chinese Herbs Such As Garlic Antibacterial Experiment – Chinese Herbal Medicine – Pharmaceutical

Garlic is one of the important condiments diet, stomach open with food, improve digestion function, and its pungent ingredient allicin on a variety of pathogenic bacteria and fungi in deep infection [1,2], in the poultry and aquaculture culture can play in terms of animal health and disease prevention to promote the growth of [3,4]; In addition, there is garlic good antioxidant, can be added to extend the shelf life of edible oil [5]; in disease prevention, Regular consumption of garlic can improve the oxidation resistance of plasma and red blood cells [6], and can lower blood pressure, prevent atherosclerosis and cardiovascular disease [7,8]; Garlic can also inhibit colon cancer cell growth [9], can also inhibit the nitrate reduction and nitrite synthesis of bacteria to prevent the occurrence of gastric cancer [10]; addition to allicin, the garlic also contains polysaccharides and other health-care component of garlic, Ginkgo biloba can be made of natural health beverage [11, 12]. Pepper is also an important day seasoning, there to promote appetite and maintaining balance of normal flora in digestive tract function; capsaicin on the E. coli, yeast and other pathogens are inhibited [13,14]. Allicin in garlic and pepper in a bacteriostatic effect of capsaicin, this does not rule out the garlic, hot pepper in the presence of other antibacterial ingredients, not entirely representative of their antibacterial activity. The experiment on decoction of pepper and garlic alcohol preparation to study the antibacterial properties, in order to improve both health-care antimicrobial properties of spices. Cork, dandelion and other common herbs used in the treatment of microbial diseases, disease control can be used as feed Additive , But they are common pathogens and antimicrobial concentrations of the antimicrobial spectrum is not clear, limited in their Medicine And feed additives in the application. Here, we explore the garlic, pepper, 12 kinds of medicinal Cork Plant Extract E. coli, paratyphoid Salmonella, Staphylococcus aureus, Bacillus subtilis and other common pathogens of the antibacterial spectrum and the minimum inhibitory concentration (MIC), hope for their medicinal use and scientific basis for production applications.

1 Materials and equipment 1.1 and medium strain of E. coli, paratyphoid Salmonella, Staphylococcus aureus, Bacillus subtilis-based laboratory retention; E. coli, paratyphoid Salmonella and Bacillus subtilis as nutrient agar medium No.3, Staphylococcus aureus Staphylococcus medium 110 agar and nutrient agar No.3.

1.2 devices Vertical Flow Clean Benches, DH3600A type electric constant temperature incubator, portable high-pressure autoclaves, 2D-85-type constant temperature oscillators, TU-1800 UV visible spectrophotometer, ZD-85-type temperature swing bed, AB204-S Basic Electronic Of days equal.

1.3 and pepper garlic herbs purchased from the farmers market, other herbs were purchased from Traditional Chinese medicine Shop.

2 Methods 2.1 allicin Extract Preparation of the peeled garlic mashed 100g small study, ethanol extraction, volatile extract was concentrated to 200ml, get 50% of garlic extracts, and twice with ethanol dilution series.

2.2 pepper, Cork and other herbal extracts prepared by decoction of Chinese herbs class crushing screening, for dry matter content (DMC) determination; the herbal powder 50g (dry weight in total) decoction preparation and concentration to 100ml, extract concentration of 50%, water do double series dilution. Antimicrobial susceptibility test disk

2.3 [15] to 6mm diameter filter paper in the herbal extracts in different concentration gradient soaked and dried, put the amount of bacteria coated with specific sub-area flat-panel, 37 overnight After the training records of all the herbal extract dilution antimicrobial gradient on the situation of several pathogens, recorded inhibition zone diameter and minimal inhibitory concentration (MIC), the qualitative and quantitative inhibition experiments were repeated 3 times.

2.4 media on the antibacterial effect of herbal Staphylococcus aureus were coated on nutrient agar agar 110 No.3 and Staphylococcus to antimicrobial susceptibility disk diffusion method to observe the Chinese herbal preparation on the two medium inhibitory effect of Staphylococcus aureus.

2.5 coating coated bacteria to determine bacteria levels in the production of 10-fold diluted liquid gradient series, to plate coating concentration of bacteria coating method.

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